All conservation methods as well as all reactivation methods lead
to the infection of soybean leaves after 1 year of storage. Regarding efficiency and labour input, the most recommended method is to tap off spores from infected and sporulating leaves with subsequent click here dehydration before storage at −80°C. Because hydration or heat shock steps did not provide any advantages, spores can be suspended in Tween water directly after storage and used as inoculum. “
“Two isolates (CVd-WHw and CVn-WHg) recovered from Verticillium-wilt-symptomatic cotton grown in Hubei Province of China were identified based on their morphology, growth characteristics in culture, specific amplification and identification Tanespimycin datasheet of internal transcribed spacer (ITS) rDNA sequence. According to the morphological characteristics, specific PCR
amplification and ITS sequences, CVd-WHw was identified as V. dahliae and CVn-WHg as Gibellulopsis nigrescens. In bioassays, the two isolates had significantly lower pathogenicity to cotton plant than V. dahliae isolate CVd-AYb. Cotton pre-inoculated with isolate CVn-WHg or CVd-WHw exhibited reduced disease indices of Verticillium wilt compared with those inoculated with CVd-AYb alone. Cotton co-inoculated with CVn-WHg or CVd-WHw and CVd-AYb provided increased protection from subsequent CVd-AYb inoculation. These results suggest that the two isolates have the potential to be developed as biocontrol agents for the control of Verticillium wilt in cotton. To our
knowledge, this is the Nintedanib (BIBF 1120) first report of a cross-protection phenomenon using Gibellulopsis nigrescens against Verticillium wilt caused by V. dahliae on cotton. “
“To identify Fusarium species associated with diseases of root and basal plate of onion, surveys were conducted in seven provinces of Turkey in 2007. Samplings were performed in 223 fields, and 332 isolates belonging to 7 Fusarium spp. were obtained. The isolates were identified as F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. proliferatum, F. redolens, and F. culmorum based on morphological and cultural characteristics. Also, species-specific primers were used to confirm the identity of Fusarium species. F. oxysporum was the most commonly isolated species, comprising 66.57% of the total Fusarium species. F. redolens was identified for the first time in onion-growing areas of Turkey. Selected isolates of each species were evaluated for their aggressiveness on onion plant. F. oxysporum, F. solani, F. acuminatum, F. proliferatum, and F. redolens were highly pathogenic, causing severe damping-off on onion plants cv. Texas Early Grano. Inter-simple sequence repeats (ISSR) markers revealed a high degree of intra- and interspecific polymorphisms among Fusarium spp. “
“The complete nucleotide sequence of an Indian isolate of Apple chlorotic leaf spot virus (ACLSV) was determined and found to be 7,525 nt in length.