Bile acids were significantly higher in patients with pruritus (p

Bile acids were significantly higher in patients with pruritus (p<0.001), particularly in those with resistant pruritus. Cortisol (p=0.02) and androsterone INCB024360 datasheet sulfate were in lower levels, while pregnenolone sulfate and an isomer of dehydroe-piandrosterone sulfate (DHEAS) were higher in patients with pruritus. Most metabolites decreased in sera after MARS and the differences were particularly significant for sterols, N-acyl ethanolamines, 1-ether,2–acylglycero-phosphoetanolamine and free sphingoid bases. MARS treatment

resulted in a significant reduction of primary bile acids (p = 0.03) and secondary bile acids, pregnenolone sulfate (p=0.007), DHEAS (p=0.02), an androsterone sulfate isomer (p=0.003), some glycero-phospholipids and kynurenine (p=0.02). Four of these serum metabolites, including bile acids were identified in the albumin dialysate. Conclusion: Cholestatic pruritus Doxorubicin is associated with increased bile acids and changes in the lipid profile. MARS therapy for pruritus results in a decrease of circulating metabolites especially phospholipids, primary bile acids and

sterols. This metabolomic analysis identifies a panel of biomarkers that could participate into the pathogenesis of cholestatic pruritus. Disclosures: Albert Pares – Consulting: Lumena Pharmaceuticals The following people have nothing to disclose: Miriam Perez-Cormenzana, Alvaro Diaz-Gonzalez, Rebeca Mayo, Azucena Castro, Antoni Mas Purose: Recently, it has been reported that the migration of inflammatory cells via high endothelial venules (HEVs) is related to the pathogenesis in various chronic inflammatory diseases. Moreover, it is known that inflammatory areas with HEVs sometimes show the characteristics of secondary lym-phoid tissue, and these MCE公司 structures are called “tertiary lymphoid organs (TLOs)”. In the present study, to examine whether the neogenesis of HEVs and the formation of TLOs are occurred in primary biliary cirrhosis (PBC), we performed the histopathological study. Methods: We examined the liver

specimens of 21 PBC cases, 13 chronic viral hepatitis-C (CVH) cases, and 5 normal cases. We performed immunohistochemistry for MECA-79, which is well-established marker of HEVs, CD3, CD20, CD21, CD83, and CCR-7. Results: In PBC livers, HEVs labeled by MECA-79 were observed more frequently in portal areas with lymphoid aggregation in PBC than in CVH (78% versus 27%; p < 0.01 Fisher’s exact test). On the other hand, HEVs were never observed in normal livers. In addition, CCR-7+ lymphocytes, which migrate to peripheral tissues via HEVs, were observed more frequently in inflammatory cites in PBC livers compared to CVH livers. Moreover, in PBC livers, HEVs were observed in 77% of portal areas with bile duct obstruction, whereas they were observed in 28% of portal areas without bile duct obstruction (p < 0.01 Fisher’s exact test). Next, we examined whether TLO’s features are recognized in 13 PBC cases, in which HEVs were remarkably observed.

Comments are closed.