Disclosures: The following people have nothing to disclose: Francesco Ridolfi, Teresa Abbattista, Annamaria Schimizzi, Eugenio Brunelli Background Nutlin-3a molecular weight & Aims: Supersonic shear-wave elastography (SWE) has not been investigated in patients with alcoholic liver disease, and there is sparse data regarding transient elastography (TE). A particular concern is whether ongoing alcohol abuse and inflammation affects liver stiffness measurements. Methods:

In two cohorts of patients with prior or current alcohol abuse the influence of METAVIR fibrosis stage, ongoing drinking, alanine transaminase (ALT), alkaline phosphatase, AB0 blood type, BMI, smoking, gender and age on SWE and TE measurements and failure rates were evaluated by regression analysis. SWE were considered a failure if JNK signaling inhibitors less than three measurements could be obtained

with a Q-box of at least 15mm and a standard deviation below 30% of the mean. Results: 252 patients were included (61% male, mean age 55 years). The majority of patients had a liver biopsy performed on the same day as the elastographies (n=141, METAVIR F0/1/2/3/4 = 31/34/19/9/48). Of the included patients, 72 were still drinking alcohol, of whom 36 were classified as abusers (>24g of alcohol per day for men and >16g/d for women). The median ALT and alkaline phosphatase were 34 U/L (interquartile range 24) and 98 U/L (interquartile range 63). There was no evidence of collinearity. In univariate regression analysis, degree of fibrosis, alcohol abstinence and level of alkaline phosphatase correlated with higher liver stiffness values measured by SWE. Degree of fibrosis and alkaline phosphatase correlated with higher TE measurements. Neither alcohol overuse nor ALT were predictors of liver stiffness. In multivariate analysis, degree of fibrosis and level of alkaline phosphatase correlated with higher liver stiffness for both SWE (fibrosis grade, coefficient 4.10, 95% CI 2.96-5.24,

P<0.001 and alkaline phosphatase, coefficient 0.06, 95% CI 0.03-0.09, P<0.001) and selleck inhibitor TE (fibrosis grade, coefficient 7.16, 95% CI 5.43-8.89, P<0.001 and alkaline phosphatase, coefficient 0.09, 95% CI 0.05-0.13, P<0.001). Alcohol abstinence, smoking and age were all correlated with higher rate of SWE failures in univariate analysis. However, the only independent predictors of failure using SWE were smoking (coefficient −0.07, 95% CI −0.14 to −0.004, P=0.039) and age (coefficient -0.004, 95% CI −0.01 to −0.00, P=0.046). BMI were the only predictor of TE failure in both uni- and multivariate analysis (coefficient −0.02, 95% CI −0.03 to −0.01, P<0.001). Conclusions: In patients with alcoholic liver disease, fibrosis grade and alkaline phosphatase influence elastography measurements using SWE or TE. Ongoing alcohol abuse does not impair liver stiffness measurements in patients with alcoholic liver disease. Disclosures: Christian P.