poae BIHB 730 5.0 ± 0.09 3.70 25.7 ± 1.4 5055.3 ± 5.0 16.4 ± 1.2 ND ND ND ND ND 5097.4 BIHB 752 7.7 ± 0.10 3.90 8.0 ± 0.8 7119.0 ± 3.8 ND ND ND ND ND 35.5 ± 3.4 7162.5 BIHB 808 7.6 ± 0.05 3.83 9.5 ± 1.3 7616.3 ± 3.5 ND ND ND selleck inhibitor ND ND 36.3 ± 3.3 7662.1 P. fluorescens BIHB 740 3.8 ± 0.05 4.00 12.7 ± 1.0 1117.7 ± 5.4 67.0 ± 2.6 164.0 ± 2.6 102.3 ± 1.5 ND ND ND 1463.7 Pseudomonas spp. BIHB 751 1.4 ± 0.03 4.20 13.9 ± 0.8 631.7 ± 4.4 255.0 ± 5.1 ND ND ND ND 4350.0 ± 2.5 5250.6 BIHB 756 9.4 ± 0.05 3.75 11.9 ± 0.8 5061.7 ± 9.4 51.7 ± 2.5 ND ND ND ND 57.7 ± 2.7 5183.0 BIHB 804 3.8 ± 0.40 4.03 12.5 ± 0.9 5839.3 ± 7.8 ND 43.2 ± 2.0 ND ND ND 41.8 ± 2.5 5936.8 BIHB 811 6.1 ± 0.05 LY2606368 in vivo 4.11 17.1 ± 1.2 4412.3 ± 5.2 138.8 ± 0.9 121.3 ± 1.5 108.0 ± 3.1 ND ND 658.1 ± 2.3 5455.6 BIHB 813 5.2 ± 0.30 4.32 12.0 ± 1.5 5971.7 ± 5.2 ND ND ND ND ND ND 5983.7 Total organic acids (μg/ml) 235.6 97392.7 549.4 599 266.4 128.7 0 5753.9 104925.7 Values are the mean of three replicates ± standard error
of the mean; ND = not detected; 2-KGA = 2-ketogluconic acid. During MRP solubilization the production of oxalic and gluconic acid was also detected for all the strains (Table 4). The production of 2-ketogluconic acid was shown by one Pseudomonas poae, P. fluorescens and four Pseudomonas spp. strains, lactic acid by five P. trivialis, one P. poae and three Pseudomonas spp. strains, succinic acid by three Pseudomonas spp. strains, formic acid by three P. trivialis and three Pseudomonas spp. strains, formic acid by P. fluorescens and three P. trivialis strains, malic acid by two P. trivialis, one P. poae, P. fluorescens
and four Pseudomonas spp. strains, and citric acid by one Pseudomonas sp. strain. Table 4 Organic acid production by fluorescent Pseudomonas during Mussoorie rock phosphate solubilization. Organic acid (μg/ml) Strain P-liberated Cyclin-dependent kinase 3 (μg/ml) Final pH Oxalic Gluconic 2-KGA Lactic Succinic Formic Citric Malic Total organic acids (μg/ml) P. trivialis BIHB 728 11.0 ± 0.3 3.52 15.1 ± 1.4 8443.3 ± 6.0 ND 44.9 ± 1.7 ND ND ND ND 8503.3 BIHB 736 13.1 ± 0.1 3.52 15.6 ± 1.4 9314.3 ± 7.4 ND ND ND ND ND ND 9329.9 BIHB 745 5.8 ± 0.3 3.63 14.8 ± 1.4 9394.0 ± 8.3 ND ND ND 84.0 ± 3.1 ND 930.0 ± 4.2 10422.8 BIHB 747 12.0 ± 0.2 3.49 16.3 ± 0.7 10016.7 ± 4.4 ND 36.8 ± 2.0 ND 70.4 ± 2.7 ND ND 10140.2 BIHB 749 8.0 ± 0.04 3.59 15.8 ± 0.7 12027.0 ± 5.7 ND ND ND ND ND ND 12042.8 BIHB 750 4.8 ± 0.4 3.67 11.7 ± 0.9 8460.0 ± 5.8 ND ND ND ND ND 32.3 ± 2.1 8504.0 BIHB 757 9.0 ± 0.04 3.63 10.6 ± 1.0 9460.0 ± 5.5 ND 39.