1 Liver inflammation is often characterized by T cell activation, Anti-infection Compound Library research buy inflammatory infiltration, and necrotic and apoptotic tissue damage accompanied by liver regeneration. Numerous proinflammatory cytokines such as tumor necrosis factor α (TNFα) or interferon-γ (IFNγ) promote tissue damage, whereas others such as interleukin (IL)-10 and IL-22 protect the liver from these harmful effects.2, 3 So far, only limited therapeutic options are available to ameliorate the long-term outcome of hepatic inflammatory disorders. Pre–B cell colony–enhancing factor (PBEF) was first identified by Samal

et al.4 in a search for novel cytokine-like molecules. The PBEF transcript was strongly up-regulated in lymphocytes by pokeweed mitogen and cycloheximide

and functionally synergized with IL-7 and stem cell factor in pre–B cell colony formation. We and others reported that PBEF preferentially activates mononuclear cells, in particular monocytes, thereby combining all features of a proinflammatory cytokine.5, 6 Beyond that, PBEF turned out to be the postulated enzyme catalyzing the rate-limiting step in nicotinamide Sunitinib adenine dinucleotide (NAD) synthesis.7, 8 NAD is a classic coenzyme with well-established roles in cellular redox reactions.9 In mammals, NAD+ biosynthesis comprises two pathways: the de novo pathway produces nicotinic acid (NA) mononucleotide by way of tryptophan and quinolinic acid. NA mononucleotide is transformed into NAD through Nam/NA mononucleotide adenylyltransferase 1/2 and NAD+ synthetase.10 The salvage pathway reuses nicotinamide (Nam), the end-product of NAD-consuming enzymes such as poly (adenosine diphosphate-ribose) polymerases (PARPs) or sirtuins

(SIRTs) .11 Nam is further converted to nicotinamide mononucleotide through nicotinamide phosphoribosyltransferase (Nampt), which in turn is converted to NAD by Nam/NA mononucleotide adenylyltransferase 1/2.12 Nampt represents the rate-limiting enzyme in this cascade.8 Most recently, PBEF’s enzymatic activity has been suggested to modulate immune functions Adenylyl cyclase by regulating NAD+ replenishment. FK866, a specific noncompetitive Nampt inhibitor, causes intracellular NAD+ shortage, specifically in activated immune cells. This leads to functional inactivity of NAD+-dependent enzymes such as PARP-1 and SIRT-6 that promote cellular activation.13, 14 Numerous studies have described an association between elevated PBEF expression with acute and chronic inflammatory conditions in humans and in mice. PBEF expression is elevated in neutrophils of septic patients preventing neutrophil apoptosis.15 PBEF has been found in diseased tissues of critically ill patients with acute lung injury.16 Its transcription is also highly elevated in a variety of chronic inflammatory conditions such as rheumatoid arthritis,17, 18 severe generalized psoriasis,19 and inflammatory bowel disease.