At a 0.5 cut-off, the positive predictive value was 0.95. Only one case was a clear false positive of AshTest due to cardiac insufficiency.Other selleck chemicals discordances could be also due to false negative of small biopsies. Conclusion:This study confirmed the performance of AshTest as a non-invasive alternative of transjugular liver biopsy in cirrhotic patients with suspected severe acute alcoholic hepatitis who need specific treatment. Disclosures: Marika Rudler – Speaking and Teaching: Gilead Sciences,

BMS, Gore, Eumedica Yen Ngo – Employment: BioPredictive Mona Munteanu – Employment: Biopredictive Thierry Poynard – Advisory Committees or Review Panels: Merck; Grant/Research Support: BMS, Gilead; Stock Shareholder: Biopredictive The following people have nothing to disclose: Sarah Mouri, Frederic Charlotte, Philippe Cluzel, Dominique Thabut Liver biopsy remains the gold standard for diagnosis of alcoholic hepatitis (AH). Selleckchem Maraviroc Herein, we use the metabolomics approach to identify plasma analytes that may correlate with diagnosis of AH and severity of liver disease in patients with AH. Methods: We recruited

patients with liver disease from single tertiary care center. The study population was divided between those with AH with cirrhosis (n=23) and those with cirrhosis with acute decompensation from etiologies other than alcohol (n=25). We used mass spectrometry to identify and measure 29 metabolic compounds in plasma samples from fasted subjects. Logistic regression analysis was performed to build a predictive model for AH. Results: After adjusting

for MELD score, compared to patients with cirrhosis with acute decompensation, those with AH were found to have significantly higher betaine levels and lower citrulline, homocitrulline, phenylalanine, tyrosine and octenoyl-carnitine. A combination of citrulline and betaine was found to provide excellent prediction accuracy for differentiation between AH and acute decompensation from etiologies other than alcohol (AUC=0.84), Figure. The plasma levels of carnitine [rho (95% CI), 0.54 (0.18, 0.91); p=0.005], homocitrulline [0.66 (0.34, 0.99); p<0.001] Protein kinase N1 and pentanoyl-carnitine [0.53 (0.16, 0.90); p=0.007] correlated with severity of liver disease in patients with AH based on MELD score. Higher levels of several biomarkers [carnitine p=0.005, butyrobetaine p=0.32, Homocitrulline p=0.002, Leucine p=0.027] were associated with higher mortality rate in AH patients. Conclusion: The levels of metabolomics plasma analytes might be used in diagnosis of AH and in determining patient prognosis. Disclosures: The following people have nothing to disclose: Ibrahim A. Hanouneh, Stephanie Marshall, Zhen Wang, Raed Dweik, Nizar N. Zein, David Grove, Laura E. Nagy, Arthur J. McCullough, Rocio Lopez, Stanley L. Hazen, J.