Spectra were acquired in reflectron mode and calibrated externally using a standard

peptide mix (Bruker Daltonics). Proteins were identified using Mascot v 2.2 (Matrix Science) with the following search parameters: database = NCBI, taxonomy = bacteria, enzyme = trypsin, this website mass tolerance = 30 ppm, missed cleavages = 1, fixed modifications = carbamidomethyl (Cys) and optional modifications = oxidation (Met). Acknowledgements We thank Dr. Masatoshi Inukai (International University of Health and Welfare, Japan) for the kind supply of globomycin and Dr. Kelly Tivendale for the supply of plasmid pVM01::TnphoA . Fundings I.S.P was supported by an International Postgraduate Research Scholarship and a Melbourne International

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