Under bloom conditions, where pH values can easily increase to 8.5 or higher, cells might, therefore, be able to maintain efficient Ci acquisition. Future research needs to investigate whether and how the assay pH governs the mode of Ci acquisition also in other coccolithophores species High Content Screening or phytoplankton taxa and how this may alter the energy budget of cells. Results from previous studies may need re-consideration in the light of our data showing strong short-term pH effects on Ci uptake of phytoplankton. Acknowledgments We thank Silke Thoms and Lena Holtz for the discussion of our data and their constructive feedback on this manuscript. This work
was supported by the European Community’s Seventh Framework Programme/ERC grant agreement
#205150, and by an Alexander Von Humboldt fellowship to PDT. Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. Electronic supplementary material Below is the link to the electronic supplementary material. Supplementary material 1 (XLSX 120 kb) References Anning T, Nimer N, Merrett M, Brownlee C (1996) Costs and benefits of calcification in coccolithophorids. J Mar Syst 9:45–564EGI-1 ic50 CrossRef Bach LT, Riebesell U, Schulz KG (2011) Distinguishing between the effects of ocean acidification and ocean carbonation in the coccolithophore Emiliania huxleyi. Limnol Oceanogr Dinaciclib 4��8C 56:2040–2050CrossRef Bach LT, Mackinder LCM, Schulz KG, Wheeler G, Schroeder DC, Brownlee C, Riebesell U (2013) Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi. New Phytol
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