FZ performed the identification and LGK-974 research buy annotation of the data, constructed the web site and wrote the manuscript. HC conducted the functional characterization based on structural information. All authors have read and approved the final submitted version of this manuscript.”
“Background Nitrosomonas europaea is a widely studied chemolithoautotrophic

ammonia oxidizing bacterium (AOB) that catalyzes the aerobic oxidation of ammonia (NH3) to nitrite (NO2 -) using carbon dioxide (CO2) as the preferred assimilative carbon source [1]. Bacteria closely related to N. europaea have been found in various natural and engineered environments indicating that they can proliferate under different PXD101 ic50 growth Torin 2 chemical structure conditions, by effectively utilizing growth substrates such as NH3 and oxygen [2–4]. The oxidative catabolic pathway of N. europaea involves NH3

oxidation to hydroxylamine (NH2OH) by membrane bound ammonia monooxygenase (AMO) and NH2OH oxidation to NO2 – by periplasmic hydroxylamine oxidoreductase (HAO) (Figure 1) [5]. In addition, autotrophic denitrification by N. europaea has also been shown [6–8]. It is believed that denitrification by N. europaea is especially favored during growth under low dissolved oxygen (DO) concentrations or high nitrite concentrations [9] and results in the production of nitric oxide (NO) or nitrous oxide (N2O) [10, 11]. However, little information Methane monooxygenase exists on the mechanisms driving the

responses of N. europaea to DO limitation and possible NO2 – toxicity [12]. For instance, it is as yet unknown whether responses to DO limitation and NO2 – toxicity at the whole-cell level are ultimate manifestations of changes in gene transcription and expression. Figure 1 Schematic of oxidative (unshaded enzymes) and reductive (gray shaded enzymes) nitrogen transformations in N. europaea (modified after [5]). In this study, the ability of N. europaea to transcribe four key genes involved in its catabolic pathway as a function of batch growth conditions (NH3 sufficiency and starvation, DO limitation and NO2 – toxicity) was evaluated. It was hypothesized that DO limitation and NO2 – toxicity would result in lower transcription of genes coding for NH3 and NH2OH oxidation (amoA and hao, respectively), given that these are the main steps leading to energy generation in N. europaea [5]. Furthermore, given that low DO and high NO2 – concentrations are two main triggers for expression of denitrification genes in heterotrophic bacteria [13], it was hypothesized that decreasing DO concentrations and high NO2 – concentrations would similarly induce progressively higher transcription of NO2 – and NO reductase genes in N. europaea (nirK and norB, respectively). The specific objectives of this study were to (i) quantitatively measure the transcription of amoA, hao, nirK and norB, four genes involved in redox N transformations, in N.