CMV-, EBV- and ADV-specific T cells were enumerated in 170 G-CSF-mobilized stem cell and 24 non-mobilized platelet donors using 14 HLA-matched multimers. T-cell

function was evaluated by IFN-gamma ELISpot and granzyme B secretion. Immunophenotyping was performed by multicolor flow cytometry. G-CSF treatment did not significantly influence frequency of antiviral T cells nor their in vitro expansion rate upon antigen restimulation. However, T-cell function was significantly impaired, as expressed by a mean reduction Lazertinib inhibitor in secretion of IFN-gamma (75% in vivo, 40% in vitro) and granzyme B (32% target-independent, 76% target-dependent) as well as CD107a expression (27%). Clinical follow up data indicate that the first CMV-reactivation in patients and with it the need for T-cell transfer occurs while the

donor is still under the influence of G-CSF. To overcome these limitations, T-cell banking before mobilization or recruitment of third party donors might be an option to optimize T-cell production.”
“We recently introduced a homogeneous immunoassay based on time-resolved Frster resonance energy transfer (TR-FRET) elicited by fluorophore-labeled antigen and fluorophore-labeled protein L, bound by an immunoglobulin. As the first clinical application, we employ this approach (LFRET) in serodiagnosis of Puumala hantavirus (PUUV) infection. A reference panel BI6727 containing serum from individuals with acute (n = 21) or past (n = 17) PUUV 123 infection and from PUUV-seronegative individuals (n = 20) was used to define the parameters. The clinical assay performance was evaluated with a prospectively collected serum panel (panel 2; n = 153). Based on the

results for panel 1, the threshold for positivity was set at a signal level that was 3-fold over background, while those with a signal smaller than 3-fold over the background level were considered PUUV seronegative. With panel 1, 20/21 acute-and 7/10 past-infection samples induced positive signals, compared to 0/20 seronegatives. With panel 2, a positive signal was obtained in 39/40 acute-and 4/10 past-infection samples, as opposed to 7/103 seronegatives. However, after IgG depletion, 58/61 acute-infection samples were LFRET positive, while all past-infection and seronegative samples were negative, corresponding to 100% 17DMAG order specificity and 95% sensitivity in detection of acute PUUV infection. We demonstrate that the novel immunoassay is a promising tool for rapid serodiagnosis of acute Puumala virus infection.”
“New series of thiourea derivatives incorporating a hippuric acid moiety have been synthesized through the reaction of 4-hippuric acid isothiocyanate with various nitrogen nucleophiles such as aliphatic amines, aromatic amines, sulfa drugs, aminopyrazoles, phenylhydrazine and hydrazides. The synthesized compounds were tested against bacterial and fungal strains.

Libraries generated following this strategy were evaluated in terms of their folding competence and their functional proficiency, an observation that was formalized as a Structure-Function Loop Adaptability value. Molecular details about the function and structure of some variants were obtained by enzyme kinetics and circular dichroism. This strategy yields functional variants that retain selleck inhibitor the original activity at higher frequencies, suggesting a new strategy for protein engineering that incorporates

a more divergent sequence 432 exploration beyond that limited to point mutations. We discuss how this approach may provide insights into the mechanism of enzyme evolution and function. (C) 2011 Elsevier Ltd. All rights reserved.”
“For clinicians, soft connective tissue integration (STI), one of the critical issues for dental implant success, is usually tested using the fibroblasts monolayer regime. Therefore, we aimed at an extension of this regime by employing interactive gingival fibroblast-keratinocyte cocultures (CCs) as an in vivo-like test platform. In the extended regime, 13 STI-relevant genes were analyzed in response to five different titanium implant biomaterial

surfaces. The genes quantitated by real-time polymerase chain reaction were categorized as pro supportive or contra supportive, that is, nonsupportive for cell growth on an engineered surface. Monocultures had higher levels of AZD7762 contra supportive gene expression, but the fibroblast-keratinocyte CC had two out of five of the titanium Dorsomorphin manufacturer surfaces with more pro supportive gene expression than contra supportive gene expression. We defined this change from contra supportive gene expression to pro supportive gene expression by developing the “relative supportive difference” index. Hence, interactive CCs exhibit valuable supportive effects on the expression of STI-relevant genes, possibly via physiological cell-to-cell-interactions. Our results render interactive gingival CCs suitable as a test platform

for dental implant-related STI under more in vivo-like conditions.”
“A study on the prevalence of hydatidosis in cattle, goats and sheep was carried out in Ngorongoro district of Arusha region, Tanzania. A 4-years data records from four slaughter slabs were retrieved and analysed. In addition, meat inspection was done in the same slaughter slabs for nine months and 64 households were interviewed to assess the community awareness on hydatidosis. Results showed the overall prevalence of hydatidosis to be 47.9%. Species prevalence of 48.7%, 34.7% and 63.8% in cattle, goats and sheep respectively was recorded. Of 174 cysts examined in cattle, 37 (21.3%) were fertile, 126 (72.4%) were sterile and 11 (6.3%) were calcified. Out of 215 goats and 67 sheep cysts examined, 52 (24.7%) and 26 (38.8%) were fertile, 138 (64.2%) and 38 (56.7%) were sterile, 24 (11.2%) and 3 (4.5%) were calcified respectively.

The secreted products interact with

hepatocytes and various immune cells in the liver. Altered liver metabolism and determinants of insulin resistance associated with visceral adipose tissue distribution are discussed, its well as, determinants of an insulin-resistant https://www.selleckchem.com/products/nu7441.html state promoted by the increased free fatty acids and cytokines delivered by visceral adipose tissue to the liver. (C) 2008 Elsevier Masson SAS. All rights reserved.”
“Coffea canephora Pierre ex Frohener is a perennial plant originated from Africa. Two main groups, Guinean and Congolese, have already been identified within this species. They correspond to main refugia in western and central Africa. In this paper we present the analysis of a region that has not yet been studied, Uganda. Two wild, one feral (once cultivated but abandoned for many years), and two cultivated populations of C. canephora from Uganda were evaluated using 24 microsatellite markers. Basic diversity, DZNeP concentration dissimilarity and genetic distances between individuals, genetic differentiation

between populations, and structure within populations were analysed. Expected heterozygosity was high for wild compartments (0.48 to 0.54) and for cultivated and feral ones (0.57 to 0.59), with the number of private alleles ranging from 12 for cultivated genotypes to 37 for a wild compartment. The Ugandan samples show significant population structuring. We compared the Ugandan populations with a representative sample of known genetic diversity groups within the species using 18 markers. Coffea canephora of Ugandan

origin was found to be genetically different from previously identified diversity groups, implying that it forms another diversity group within the species. Given its large distribution and extremely recent domestication, C. canephora can be used to understand the effect of refugia colonization on genetic diversity.”
“Background: Elderly patients with ST-elevation myocardial infarction (STEMI) are often underrepresented in major percutaneous coronary intervention (PCI) trials. check details Use of PCI for STEMI, and associated outcomes in patients aged >= 65 years with STEMI needed further investigation.\n\nMethods: We used the 2001-2010 United States Nationwide Inpatient Sample (NIS) database to examine the temporal trends in STEMI, use of PCI for STEMI, and outcomes among patients aged 65-79 and >= 80 years.\n\nResults: During 2001-2010, of 4,017,367 patients aged >= 65 years with acute myocardial infarction (AMI), 1,434,579 (35.7%) had STEMI. Over this period, among patients aged 65-79 and >= 80 years, STEMI decreased by 16.4% and 19%, whereas the use of PCI for STEMI increased by 33.5% and 22%, respectively (Ptrend 0.001). There was a significant 3 decrease in age-adjusted in-hospital mortality (per 1000) in patients aged >= 80 years (150 versus 116, P-trend – 0.02) but not in patients aged 65-79 years (63 versus 59, P-trend – 0.886).

“
“We report results from a detailed computer simulation study for the nano-sorption and mobility of four different small molecules (water, tyrosol, vanillic acid, and p-coumaric acid) inside smooth single-wall carbon nanotubes (SWCNTs). Most of the results have been obtained with the molecular dynamics (MD) method, but especially for the most 4 narrow of the CNTs considered,

the results for one of the molecules addressed here (water) were further confirmed through an additional Grand Canonical (mu VT) Monte Carlo (GCMC) simulation using a value for the water chemical potential mu pre-computed with the particle deletion method. Issues addressed include www.selleckchem.com/products/dorsomorphin-2hcl.html molecular packing and ordering inside the nanotube for the four molecules, average number of sorbed molecules per unit length of the tube, and mean residence time and effective axial diffusivities, all as a function of tube diameter and tube length. In all cases, a strong dependence of the results on tube diameter was observed, especially in the way the different molecules are packed and organized inside the CNT. For water for which predictions of properties such as local structure and packing were computed

with both methods (MD and GCMC), the two sets of results were found to be fully self-consistent for all types of SWCNTs considered. Water diffusivity inside the CNT (although, strongly dependent on the CNT diameter) was computed with two different methods, both of ABT-737 research buy which gave identical results. For large enough CNT diameters (larger than about 13 angstrom), this was found to be higher than the corresponding experimental value in the bulk by about 55%. Surprisingly enough, for the rest of the molecules

simulated (phenolic), the simulations revealed no signs of mobility inside nanotubes with a diameter smaller than the (20, 20) tube. This is attributed to strong phenyl-phenyl attractive interactions, also to favorable interactions of these molecules with the CNT walls, which cause selleck chemicals llc them to form highly ordered, very stable structures inside the nanotube, especially under strong confinement. The interaction, in particular, of the methyl group (present in tyrosol, vanillic acid, and p-coumaric acid) with the CNT walls seems to play a key role in all these compounds causing them to remain practically immobile inside nanotubes characterized by diameters smaller than about 26 angstrom. It is only for larger-diameter CNTs that tyrosol, vanillic acid, and p-coumaric acid were observed to demonstrate appreciable mobility. (C) 2013 AIP Publishing LLC.”
“Process control of protein therapeutic manufacturing is central to ensuring the product is both safe and efficacious for patients. In this work, we investigate the cause of pink color variability in development lots of monoclonal antibody (mAb) and Fc-fusion proteins.

The results revealed that the left dorsolateral prefrontal

cortex (DLPFC) was more active in the higher-order switching, and the right fronto-polar cortex (FPC) was significantly activated with the lower-order switching. We discuss a possible functional model Cl-amidine in the prefrontal cortex where the left DLPFC encodes the hierarchical organization of behaviours and the right FPC maintains and updates multiple behavioural. This interpretation is highly consistent with the previous findings and current theories of hierarchical organization in the prefrontal functional network. (C) 2009 Elsevier Inc. All 3 rights reserved.”
“Dentinal proteases are believed to play an important role in the degradation of hybrid layers (HL). This study investigated the HL gelatinolytic activity by in situ zymography and functional enzyme activity assay. The hypotheses were that HLs created by an etch-and-rinse adhesive exhibit active gelatinolytic activity, and MMP-2 and

-9 activities in dentin increase during adhesive GDC-0068 in vitro procedures. Etched-dentin specimens were bonded with Adper Scotchbond 1XT and restored with composite. Adhesive/dentin interface slices were placed on microscope slides, covered with fluorescein-conjugated gelatin, and observed with a multi-photon confocal microscope after 24 hrs. Human dentin powder aliquots were prepared and assigned to the following treatments: A, untreated; B, etched with 10% phosphoric acid; or C, etched with 10% phosphoric acid and mixed with Scotchbond 1XT. The MMP-2 and -9 activities of extracts of dentin powder were measured with functional enzyme assays. Intense and continuous enzyme activity was detected at the bottom of the HL, while that activity was more irregular in the upper HL. Both acid-etching and subsequent adhesive application significantly increased MMP-2 and -9 activities (p < 0.05). The results demonstrate, for the first time, intrinsic MMP activity in the HL, and intense activation

of matrix-bound MMP activity with both etching and adhesive application.”
“Tonic immobility (TI) is a reversible coma-like stasis inherent to a variety of terrestrial and aquatic taxa, including elasmobranchs, yet virtually nothing is known about Selleck VS-4718 its underlying neurological and physiological processes in any taxa. The purpose of this research was to investigate the physiological effects of TI on the juvenile lemon shark (Negaprion brevirostris). Eight juvenile lemon sharks were subjected to four, three-hour treatments during which blood was sampled at 0, 30,90 and 180 min, over a 6 week period. Treatments were differentiated by the method of maintaining the shark, either in TI, or allowed to swim freely between blood samples and the presence or absence of a pre-treatment exercise period designed to simulate the capture induced exhaustion that usually precedes the use TI in the field.

Finally, the similarities between different ciliopathies at the phenotypic level are proving to be due to their shared cellular defect and also their common genetic basis. To this end, recent studies are showing that mutations in a given ciliary gene often appear involved in the pathogenesis of more than one clinical entity, complicating their genetic dissection, and hindering our ability to generate accurate genotype-phenotype correlations. (C) 2009 Wiley-Liss, Inc.”
“A full description of the human proteome relies on the challenging task of detecting mature and changing forms of protein molecules in the

body. Large-scale proteome analysis(1) has routinely involved digesting intact proteins followed by inferred protein identification ACY-738 using mass spectrometry(2). This ‘bottom-up’ process affords a high number of identifications (not always unique to a single gene). However, 4 complications arise from incomplete or ambiguous(2) characterization of alternative splice forms, diverse modifications (for example, acetylation and methylation) and endogenous protein cleavages, especially when combinations of these create complex patterns of intact protein isoforms and species(3). ‘Top-down’

interrogation of whole proteins can overcome these problems for individual proteins(4,5), find more but has not been achieved on a proteome scale owing to the lack of intact protein fractionation methods that are well integrated with tandem mass spectrometry. Here we show, using a new four-dimensional separation system, identification of 1,043 gene products from human cells that are dispersed into more than 3,000 protein species created by post-translational modification (PTM), RNA splicing and proteolysis. The overall system produced greater than 20-fold increases in both separation power and proteome coverage, enabling the identification of proteins up to 105 kDa and those with up to 11 transmembrane

helices. Many previously undetected isoforms of endogenous human proteins were mapped, including changes in multiply modified species Selleck Napabucasin in response to accelerated cellular ageing (senescence) induced by DNA damage. Integrated with the latest version of the Swiss-Prot database(6), the data provide precise correlations to individual genes and proof-of-concept for large-scale interrogation of whole protein molecules. The technology promises to improve the link between proteomics data and complex phenotypes in basic biology and disease research(7).”
“Reduced expression of dyskinesia is observed in levodopa-primed MPTP-treated common marmosets when dopamine agonists are used to replace levodopa. We now investigate whether a combination of the D-2/D-3 agonist pramipexole and levodopa also reduces dyskinesia intensity while maintaining the reversal of motor disability. Drug naive, non-dyskinetic MPTP-treated common marmosets were treated daily for up to 62 days with levodopa (12.5 mg/kg plus carbidopa 12.5 mg/kg p.o. BID) or pramipexole (0.04-0.

Retrospective review on prospective cohort and explicit chart review.\n\nObjective. To identify early spine 4 trauma predictors of functional disability and to assess management

compliance to established spine trauma treatment algorithms.\n\nSummary of Background Data. Identification of early (within 48 hours) spine trauma predictors of functional disability is novel and may assist in the management of patients with trauma. Also, with significant global variation, spine trauma treatment algorithms are essential.\n\nMethods. Analysis was performed on patients with spine HKI-272 purchase trauma from May 1, 2009, to January 1, 2011. Functional outcomes were determined using the Glasgow Outcome Scale (GOS) at 1 year. Univariate and multivariate regressions were applied to investigate the effects of the injury severity score, age, blood sugar level, vital signs, traumatic brain injury, comorbidities, coagulation profile, neurology, and spine injury characteristics. ASP2215 A compliance study was performed using the SLIC and TLICS spine trauma algorithms.\n\nResults. The completion rate for the GOS was 58.8%. The completed GOS cohort was 4.2 years younger in terms of mean age, had more number of patients with severe polytrauma, but less number of patients with

severe spinal cord injuries (ASIA [American Spinal Injury Association] A, B, and C) in comparison with the uncompleted GOS cohort. Multivariate logistic regression revealed 3 independent early spine trauma predictors of functional disability with statistical significance (P < 0.05). They were (1) hypotension (OR [odds ratio] = 1.98; CI [confidence interval] = 1.13-3.49), (2) hyperglycemia (OR = 1.67; CI = 1.09-2.56), and (3) moderate/severe traumatic brain injury (OR https://www.selleckchem.com/products/cx-4945-silmitasertib.html = 5.88; CI = 1.71-20.16). There were 305 patients with subaxial cervical spine injuries and 653 patients with thoracolumbar spine injuries. The subaxial cervical spine injury classification and thoracolumbar injury classification and severity score compliance studies returned agreements of 96.1%

and 98.9%, respectively.\n\nConclusion. Early independent spine trauma predictors of functional disability identified in a level 1 trauma center with high compliance to the subaxial cervical spine injury classification and thoracolumbar injury classification and severity score algorithms were hypotension, hyperglycemia, and moderate or severe traumatic brain injury. Spine trauma injury variables alone were shown not to be predictive of functional disability.”
“Purpose The protease inhibitor bortezomib attenuates the action of NF-kappa B and has shown preclinical activity alone and in combination with chemotherapy. Design A Phase I dose-escalation study was performed administering bortezomib (0.7, 1.0, 1.3 and 1.

In summary, the different chemical properties of CPPs have little correlation with their ability to efficiently deliver splice-correcting PNA. However, conjugates of polycationic

and amphipathic peptides appear to utilize different internalization routes.”
“Retroviral vectors integrate in genes and regulatory elements and may cause transcriptional deregulation of gene expression in target cells. Integration into transcribed genes also has the potential to deregulate gene expression at the posttranscriptional level by interfering with splicing and polyadenylation of primary transcripts. To examine the impact of retroviral vector integration on transcript splicing, we transduced primary human cells or cultured cells with HIV-derived vectors carrying a reporter gene or a human beta-globin gene under the control of a reduced-size locus-control region (LCR). selleck inhibitor Cells were randomly cloned

and integration sites were determined in individual clones. We identified aberrantly spliced, chimeric transcripts in more than half of the targeted genes in all cell types. Chimeric transcripts were generated through the use of constitutive and cryptic splice sites in the HIV 5′ long terminal repeat and gag gene as well as in the beta-globin gene and P005091 LCR. Compared with constitutively spliced transcripts, most aberrant transcripts accumulated at a low level, at least in part as a consequence of nonsense-mediated. mRNA degradation. A limited set of cryptic splice MX69 cost sites caused the majority of aberrant splicing events, providing a strategy

for recoding lentiviral vector backbones and transgenes to reduce their potential posttranscriptional genotoxicity.”
“Bromine radical-mediated cyclopropylcarbinyl-homoallyl rearrangement of alkylidenecyclopropanes was effectively accomplished by C-C bond formation with allylic bromides, which led to the syntheses of 2-bromo-1,6-dienes. A three-component coupling reaction comprising alkylidenecyclopropanes, allylic bromides, and carbon monoxide also proceeded well to give 2-bromo-1,7-dien-5-ones in good yield.”
“The title amine, C13H14N2, is twisted with a dihedral angle between the rings of 60.07 (9)degrees. The amine N-H group and pyridine N atom are syn allowing for the formation of centrosymmetric eight-membered center dot center dot center dot HNCN(2) synthons via N-H center dot center dot center dot N hydrogen bonds. The two-molecule aggregates are sustained in the three-dimensional crystal packing via C-H center dot center dot center dot pi and pi-pi interactions [centroid-centroid distance for pyridyl rings = 3.7535 (12) angstrom]“
“Objectives: (1) to investigate the test-retest reliability of 3D gait analysis (3DGA) in hip Osteoarthritis (OA) patients; (2) to find the minimum number of gait trials needed to overcome intrinsic variability; (3) to check the accuracy of angles measured by the 3D system.

\n\nRESULTS\n\nThe quantitative morphometric analysis was technically successful in all cases. The mean parameters were as follows: compactness 0.88 +/- 0.086, MRD 0.83 +/- 0.056, RDSD 0.087 +/- 0.037, RDAR 0.045 +/- 0.023, zero crossings 6 +/- 2.2, entropy 1.43 +/- 0.16, MFD 4.40 +/- 3.14 cm, Feret ratio 0.78 +/-

0.089, CHA 0.98 +/- 0.027, CHP 0.98 +/- 0.030, EC 0.95 +/- 0.043, and El 0.95 +/- 0.023. MFD and RDAR provided the widest value range for the best shape discrimination. The larger tumors www.selleckchem.com/products/gant61.html were less compact, more concave, and less ellipsoid than the smaller tumors (P<0.0001). AFP-producing tumors displayed greater morphologic irregularity based on several parameters, including compactness, MRD, RDSD, RDAR, entropy, and El (P<0.05 for all).\n\nCONCLUSION\n\nComputerized HCC image analysis using shape descriptors is technically feasible. Aggressively growing tumors have wider diameters and more irregular margins. Future studies will determine further clinical applications for this morphologic analysis.”
“Background: Microfibrillar-associated protein 4 (MFAP4)

is a systemic biomarker that see more is significantly elevated in samples from patients suffering from hepatic cirrhosis. The protein is generally localized to elastic fibers and other connective tissue fibers in the extracellular matrix (ECM), and variation in systemic MFAP4 (sMFAP4) has the potential JQ1 nmr to reflect diverse diseases with increased ECM turnover. Here, we aimed to validate an enzyme-linked immunosorbent assay (ELISA) for the

measurement of sMFAP4 with an emphasis on the robustness of the assay. Moreover, we aimed to determine confounders influencing the basal sMFAP4 variability and the genetic contribution to the basal variation. Methods: The sandwich ELISA was based on two monoclonal anti-MFAP4 antibodies and was optimized and calibrated with a standard of recombinant MFAP4. The importance of pre-analytical sample handling was evaluated regarding sample tube type, time, and temperature conditions. The mean value structure and variance structure was determined in a twin cohort including 1,417 Danish twins (age 18-67 years) by mixed-effect linear regression modeling. Results: The practical working range of the sandwich ELISA was estimated to be 4-75 U/ml. The maximum intraand inter-assay variation was estimated to be 8.7% and 6.6%, respectively. Sample handling and processing appeared to influence MFAP4 measurements only marginally. The average concentration of sMFAP4 in the serum was 18.9 +/- 8.4 (SD) U/ml in the twin cohort (95% CI: 18.5-19.4, median sMFAP4 17.3 U/ml). The mean structure model was demonstrated to include waist-hip ratio, age, and cigarette smoking status in interactions with gender. A relatively low heritability of h(2) = 0.24 was found after applying a model including additive genetic factors and shared and non-shared environmental factors.

Among the

members of this prohormone convertase family, Neuroendocrine Convertase-2 (NEC-2) is regarded as one of the important proteins involved in the maturation of many precursor proteins. Being widely distributed in the neuroendocrine cells, these proteins play a vital role in causing malignant gliomas. They can serve as important drug targets in the treatment of cancers. In the present study, a 3D model of NEC-2 was generated using homology modeling. The model was optimized by a brief energy minimization in CHARMM and dynamics simulation of 250ps in MOE. The validation results of PROCHECK and Profile 3D show that the stereochemical quality of the model is good. The C alpha backbone of the template and the target (NEC-2) when superimposed showed RMSD of 0.39

angstrom. The model showed Asp51, His92 and Ser268 in the https://www.selleckchem.com/products/MK-2206.html active site as seen in most of the PC2 members. The NEC-2 structure differs from that of furin at the catalytic pocket region with relevance to the amino acid composition which can be exploited for the design of specific inhibitors towards NEC-2.”
“Purpose: The Raine Eye Health Study (REHS) was conceived to determine the 123 prevalence of and risk factors for eye disease in young adults, and to characterize ocular biometric parameters in a young adult cohort. This article summarizes XMU-MP-1 concentration the rationale and study design of REHS and outlines the baseline prevalence of ophthalmic disease in this population.\n\nMethods: The Western Australian Pregnancy Cohort (Raine) Study originated as a randomized-controlled trial of 2900 women recruited from the state’s largest maternity hospital. Their offspring (N = 2868) have been followed at birth, ages 1, 2, 3, 5, 8, 10, 14, 17 and 20 years of age in a prospective cohort study. DNA has been collected from participants for genome-wide association studies. At the 20-year follow-up participants completed a comprehensive eye assessment that included visual acuity, orthoptic assessment and cycloplegic autorefraction, as well as several ocular biometric variables and multiple ophthalmic photographs of the anterior and posterior segments.\n\nResults: A total of 1344 participants

(51.3% male) were assessed over a 24-month period. For the majority of examined participants (85.5%) both parents were Caucasian, Pevonedistat 63.3% had completed school year 12 or equivalent, 5.5% had myopia (spherical equivalent <=-3 diopters) and 15 participants (1.2%) had unilateral or bilateral pterygia. Keratoconus, cataract, keratitis and uveitis were rare.\n\nConclusion: The REHS design and methodology allow comparison with other population-based studies of eye disease. The study established the prevalence of eye disorders in a large sample of predominantly Caucasian young Australian adults.”
“Pasteurellosis is one of the most prevalent diseases of sheep, but the involvement of Pasteurellae in genital pathology of rams has been described rarely.